Applications

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Genetic Manipulation of Cardiomyocytes

The ability to interrogate and monitor gene expression is critical to understanding biological pathways that underlie normal and pathogenic cellular function.

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Genetic Manipulation of Cardiomyocytes

Discovery, Regenerative Medicine, Toxicity

The ability to interrogate and monitor gene expression is critical to understanding biological pathways that underlie normal and pathogenic cellular function. CDI’s cardiomyocytes are amenable to various genetic manipulation techniques including transfection, transduction, siRNA, and reporter vector expression.

Transfection and Transduction:

  1. iCell Products: Applying Transfection Technologies to Create Novel Screening Models. Cellular Dynamics Application Note.
  2. Robers M and Jarecki B. (2014) Efficiently Build Relevant In Vitro Models Using Human Stem Cell-derived Tissue Cells, High Performance Transfection and Novel Multiplexed Reporter Techniques. Promega/Cellular Dynamics Webinar.
  3. Anson B. (2015) Building Richer Assays: iPSC-derived Tissue Cells Are a Powerful Addition to the Biologist’s Tool Box. GEN 35(2).
  4. Traister A, Li M, et al. (2014) Integrin-linked Kinase Mediates Force Transduction in Cardiomyocytes by Modulating SERCA2a/PLN Function. Nature Comm 5:4533.
  5. Fine M, Lu F, et al. (2013) Human Induced Pluripotent Stem Cell-derived Cardiomyocytes for Studies of Cardiac Ion Transporters. Am J Physiol Cell Physiol 305(5):C481-91.

siRNA:

  1. iCell Products: Applying Transfection Technologies to Create Novel Screening Models. Cellular Dynamics Application Note.
  2. Traister A, Li M, et al. (2014) Integrin-linked Kinase Mediates Force Transduction in Cardiomyocytes by Modulating SERCA2a/PLN Function. Nature Comm 5:4533.
  3. Fine M, Lu F, et al. (2013) Human Induced Pluripotent Stem Cell-derived Cardiomyocytes for Studies of Cardiac Ion Transporters. Am J Physiol Cell Physiol 305(5):C481-91.

Reporter Vector Expression:

  1. Robers M and Jarecki B. (2014) Efficiently Build Relevant In Vitro Models Using Human Stem Cell-derived Tissue Cells, High Performance Transfection and Novel Multiplexed Reporter Techniques. Promega/Cellular Dynamics Webinar.

Genetic Manipulation of Neurons

The ability to interrogate and monitor gene expression is critical to understanding biological pathways that underlie normal and pathogenic cellular function.

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Genetic Manipulation of Neurons

Discovery, Regenerative Medicine, Toxicity

The ability to interrogate and monitor gene expression is critical to understanding biological pathways that underlie normal and pathogenic cellular function. CDI’s neurons and dopaneurons are amenable to various genetic manipulation techniques including:

  • Transfection
  • Transduction
  • siRNA
  • Reporter vector expression

Genetic Manipulation of Hepatocytes

The ability to interrogate and monitor gene expression is critical to understanding biological pathways that underlie normal and pathogenic cellular function.

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Genetic Manipulation of Hepatocytes

Discovery, Regenerative Medicine, Toxicity

The ability to interrogate and monitor gene expression is critical to understanding biological pathways that underlie normal and pathogenic cellular function. CDI has evaluated various genetic manipulation tools to enable the development of assays using its hepatocytes.

Genetic Manipulation of Endothelial Cells

The ability to interrogate and monitor gene expression is critical to understanding biological pathways that underlie normal and pathogenic cellular function.

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Genetic Manipulation of Endothelial Cells

Discovery, Regenerative Medicine, Toxicity

The ability to interrogate and monitor gene expression is critical to understanding biological pathways that underlie normal and pathogenic cellular function. CDI has worked to evaluate a wide range of genetic manipulation tools to enable the development of assays using its endothelial cells.