Applications

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Advanced Cardiomyocyte Cell Culture

Advanced cell culture techniques including 3D spheroids, micropatterned co-culture, bioengineered and flow-based systems, and bioprinting offer the potential to better mimic in vivo tissue structure and function.

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Advanced Cardiomyocyte Cell Culture

Discovery, Regenerative Medicine, Toxicity

Advanced cell culture techniques including 3D spheroids, micropatterned co-culture, bioengineered and flow-based systems, and bioprinting offer the potential to better mimic in vivo tissue structure and function. CDI’s cardiomyocytes are amenable to these culture techniques as pure cell populations or in co-culture with other cell types, such as CDI’s iCell Endothelial Cells.

  1. Carlson C, Einhorn S, et al. (2013) Applications Development at CDI: Improving Workflows, Pushing Biology, and Enabling Screening. Poster Presentation, Cellular Dynamics User Group Meeting.
  2. Rao C, Prodromakis T, et al. (2013)  The Effect of Microgrooved Culture Substrates on Calcium Cycling of Cardiac Myocytes Derived from Human Induced Pluripotent Stem Cells. Biomaterials 34(10):2399-411.
  3. iCell Cardiomyocytes – iCell Endothelial Cells Co-culture. Contact Technical Support for more information.

Genetic Manipulation of Cardiomyocytes

The ability to interrogate and monitor gene expression is critical to understanding biological pathways that underlie normal and pathogenic cellular function.

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Genetic Manipulation of Cardiomyocytes

Discovery, Regenerative Medicine, Toxicity

The ability to interrogate and monitor gene expression is critical to understanding biological pathways that underlie normal and pathogenic cellular function. CDI’s cardiomyocytes are amenable to various genetic manipulation techniques including transfection, transduction, siRNA, and reporter vector expression.

Transfection and Transduction:

  1. iCell Products: Applying Transfection Technologies to Create Novel Screening Models. Cellular Dynamics Application Note.
  2. Robers M and Jarecki B. (2014) Efficiently Build Relevant In Vitro Models Using Human Stem Cell-derived Tissue Cells, High Performance Transfection and Novel Multiplexed Reporter Techniques. Promega/Cellular Dynamics Webinar.
  3. Anson B. (2015) Building Richer Assays: iPSC-derived Tissue Cells Are a Powerful Addition to the Biologist’s Tool Box. GEN 35(2).
  4. Traister A, Li M, et al. (2014) Integrin-linked Kinase Mediates Force Transduction in Cardiomyocytes by Modulating SERCA2a/PLN Function. Nature Comm 5:4533.
  5. Fine M, Lu F, et al. (2013) Human Induced Pluripotent Stem Cell-derived Cardiomyocytes for Studies of Cardiac Ion Transporters. Am J Physiol Cell Physiol 305(5):C481-91.

siRNA:

  1. iCell Products: Applying Transfection Technologies to Create Novel Screening Models. Cellular Dynamics Application Note.
  2. Traister A, Li M, et al. (2014) Integrin-linked Kinase Mediates Force Transduction in Cardiomyocytes by Modulating SERCA2a/PLN Function. Nature Comm 5:4533.
  3. Fine M, Lu F, et al. (2013) Human Induced Pluripotent Stem Cell-derived Cardiomyocytes for Studies of Cardiac Ion Transporters. Am J Physiol Cell Physiol 305(5):C481-91.

Reporter Vector Expression:

  1. Robers M and Jarecki B. (2014) Efficiently Build Relevant In Vitro Models Using Human Stem Cell-derived Tissue Cells, High Performance Transfection and Novel Multiplexed Reporter Techniques. Promega/Cellular Dynamics Webinar.

Bioengineering Cardiac Tissues

CDI's cardiomyocytes are being applied in research aimed to develop and test a bioengineered, implantable cardiac patch for the treatment of heart failure.

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Bioengineering Cardiac Tissues

Regenerative Medicine

CDI’s cardiomyocytes are being applied in research aimed to develop and test a bioengineered, implantable cardiac patch for the treatment of heart failure. Initial studies demonstrate that the cardiac patches beat spontaneously and synchronously, respond to electrical stimulation, exhibit typical morphology, and improve cardiac function in rats with chronic heart failure.

  1. Lancaster J. (2012) Development and Testing of a Cardiomyocyte Scaffold for the Treatment of Heart Failure. Cellular Dynamics User Group Meeting.