Applications

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Modeling Botulinum Neurotoxin Infection

CDI's neurons provide a functionally relevant human model to measure Clostridium botulinum neurotoxin (BoNT) activity.

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Modeling Botulinum Neurotoxin Infection

Discovery, Disease Modeling, Toxicity

CDI’s neurons provide a functionally relevant human model to measure Clostridium botulinum neurotoxin (BoNT) activity. Compared with primary rat spinal cord cells, CDI’s neurons showed equal or increased sensitivity, improved dose-response, and more complete SNARE protein cleavage in response to BoNT treatment. CDI’s neurons are rapidly being adopted by researchers to study mechanisms of BoNT toxicity and by BoNT manufacturers to replace an expensive and labor-intensive mouse bioassay for potency testing.

Genetic Manipulation of Endothelial Cells

The ability to interrogate and monitor gene expression is critical to understanding biological pathways that underlie normal and pathogenic cellular function.

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Genetic Manipulation of Endothelial Cells

Discovery, Regenerative Medicine, Toxicity

The ability to interrogate and monitor gene expression is critical to understanding biological pathways that underlie normal and pathogenic cellular function. CDI has worked to evaluate a wide range of genetic manipulation tools to enable the development of assays using its endothelial cells.

Modeling Epilepsy

Epilepsy is a condition with recurring seizures caused by abnormal electrical activity in the brain.

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Modeling Epilepsy

Discovery, Disease Modeling

Epilepsy is a condition with recurring seizures caused by abnormal electrical activity in the brain. CDI’s neurons have been used to develop in vitro models that recapitulate the functional phenotype of pathogenic mutations. These models are being used to better understand the biophysical properties of ion channels with the goal of identifying candidate therapeutic molecules for improved drug safety.

  1. Padilla KM, Antonio BM, et al. (2014) Approaches to Understanding Human Ion Channel Genetic Variation and Disease – An Example with a KCNT1 Variant and Infantile Epilepsy Disorder. Poster Presentation, Society for Neuroscience.

Modeling Parkinson’s Disease

Parkinson's disease is the result of a progressing degeneration of dopamine-producing brain cells, specifically midbrain dopaminergic neurons, that results in a loss of motor function and in dementia.

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Modeling Parkinson’s Disease

Discovery, Disease Modeling

Parkinson’s disease is the result of a progressing degeneration of dopamine-producing brain cells, specifically midbrain dopaminergic neurons, that result in a loss of motor function and in dementia. CDI’s neurons and dopaneurons are being used to elucidate the mechanisms that underlie the pathogenesis of Parkinson’s disease including mitochondrial dysfunction, synapse degeneration, ubiquitin-proteasome degradation, oxidative stress, and others.

Modeling Alzheimer’s Disease

Alzheimer's disease (AD) is characterized by the neuropathological hallmarks of amyloid plaques and neurofibrillary tangles that eventually result in neuronal loss in the cerebral cortex and hippocampus.

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Modeling Alzheimer’s Disease

Discovery, Disease Modeling

Alzheimer’s disease (AD) is characterized by the neuropathological hallmarks of amyloid plaques and neurofibrillary tangles that eventually result in neuronal loss in the cerebral cortex and hippocampus. As functional human models, CDI’s neurons are actively being applied in research to investigate relevant molecular and cellular mechanisms of AD. These in vitro cellular models are capable of recapitulating the disease phenotype and have been employed in various assays including a high-throughput phenotypic screening assay resulting in the identification of candidate protective molecules.

  1. Chai X, Dage JL, et al. (2012) Constitutive Secretion of Tau Protein by an Unconventional Mechanism. Neurobiol Dis 48(3):356-366.
  2. Xu X, Lei Y, et al. (2013) Prevention of ß-amyloid Induced Toxicity in Human iPS Cell-derived Neurons by Inhibition of Cyclin-dependent Kinases and Associated Cell Cycle Events. Stem Cell Res 10(2):213-227.
  3. Maloney JA, Bainbridge T, et al. (2014) Molecular Mechanisms of Alzheimer’s Disease Protection by the A673T Allele of the Amyloid Precursor Protein. J Biol Chem 289(45):30990-1000.
  4. Alhebshi AH, Odawara A, et al. (2014) Thymoquinone Protects Cultured Hippocampal and Human Induced Pluripotent Stem Cells-derived Neurons against α-synuclein-induced Synapse Damage. Neurosci Lett 570:126-131.
  5. Carlson C, Wang J, et al. (2014) Characterization of an Isogenic Disease Model of Alzheimer’s Disease from Human iPSC-derived Neurons. Poster Presentation, Society for Neuroscience.
  6. Usenovic M, Niroomand S, et al. (2014) Model of Tau Pathology in Induced Pluripotent Stem Cell-derived Human Neurons. Poster Presentation, Society for Neuroscience.

Modeling Autism Spectrum Disorder

Autism spectrum disorder (ASD) is a group of developmental disabilities including Rett and Asperger’s syndromes that result in significant social, communication, and behavioral challenges.

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Modeling Autism Spectrum Disorder

Discovery, Disease Modeling

Autism spectrum disorder (ASD) is a group of developmental disabilities that result in significant social, communication, and behavioral challenges. Model ASD using CDI’s iPSC-derived neurons, including those from donors with autism, Asperger’s, or cell types that display autistic-like phenotypes such as Rett syndrome knock-out model.

3D Spheroid Cell Culture

3D spheroid culture of iCell Hepatocytes 2.0 for enhanced functional maturity and prolonged culturability.

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3D Spheroid Cell Culture

Discovery, Regenerative Medicine, Toxicity

Moving beyond traditional static-plated culture yields a more liver-like environment for hepatocyte assays and generates more predictive biology in vitro. The combination of iPSC technology with advanced culture techniques offers advantages over existing models.  Conditions developed allow for a tunable spheroid size with maintenance of viability and put the control over engineered tissue in the hands of the user. This novel workflow allows for the generation of iCell® Hepatocytes 2.0 microtissues in low-attachment plates.

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