Excitatory Cortical Neurons (iCell GlutaNeurons) Derived from Human iPS Cells Create Functional Macro Networks in vitro

Excitatory Cortical Neurons (iCell GlutaNeurons) Derived from Human iPS Cells Create Functional Macro Networks in vitro

Excitatory Cortical Neurons (iCell GlutaNeurons) Derived from Human iPS Cells Create Functional Macro Networks in vitro

  • Date: September 20, 2016
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The ability to produce human neuronal populations from iPS cells combined with advancements in micro electrode array (MEA) instrumentation make it now possible to study human neuronal network activity in vitro. This poster presents data demonstrating the functional neuronal network properties of iCell GlutaNeurons, a human iPSC-derived excitatory cortical neuron population that enables electrophysiology and excitatory toxicity assays. Using single cell gene expression as a guide, we established a robust differentiation process starting from iPSCs that generates primarily cortical glutamatergic neurons. iCell GlutaNeurons react to increasing amounts of glutamic acid with increased cell death exhibiting excitatory toxicity. Pre-treatment of iCell GlutaNeurons with the NMDA and AMPA receptor inhibitors, AP5 and DNQX, inhibited excitatory toxicity. Most importantly, the cells show a robust formation of a synaptically-driven macro network over time with spontaneous, synchronous electrical activity in the MEA platform. The synchronous activity can be reversibly inhibited by AP5 and DNQX, thus demonstrating the ability to modulate iCell GlutaNeurons electrophysiological activity using pharmacology.

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