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Highlights of 2015 in iCell® Cardiomyocytes Publications

A Banner Year for Publications

2015 brought an accelerated pace of peer-reviewed publications where iCell®  Cardiomyocytes played a central role across a broad range of application areas including:

  • Basic cardiomyocyte characterization
  • Complex culture methods inducing 3D spheroid and ECM scaffold methods
  • Non-invasive, label-free interrogation of cardiomyocyte activity with multiple endpoints
  • Phenotypic screening, disease modeling,  and other drug discovery applications
  • Food additive testing, general cardiotoxicity, gene modulation of cardiotoxic compounds, and surveys of compounds and compound libraries
See the Overview

See the list of publications in the table below and download the technical overview of the publications for more information on the exciting developments incorporating iCell Cardiomyocytes during 2015.

Cardiomyocyte Characterization

Title Authors Citation
Characterization of a Novel KCNQ1 Mutation for Type 1 Long QT Syndrome and Assessment of the Therapeutic Potential of a Novel IKs Activator Using Patient-specific Induced Pluripotent Stem Cell-derived Cardiomyocytes Ma D, Wei H, Lu J, Huang D, Liu Z, Loh LJ, Islam O, Liew R, Shim W, Cook SA Stem Cell Res Ther. 2015 Mar 19;6:39
Structural Phenotyping of Stem Cell-derived Cardiomyocytes Pasqualini FS, Sheehy SP, Agarwal A, Aratyn-Schaus Y, Parker KK. Stem Cell Reports. 2015 Mar 10;4(3):340-7

Culture Systems and Regenerative Medicine

Title Authors Citation
Development and Characterization of a Scaffold-free 3D Spheroid Model of Induced Pluripotent Stem Cell-derived Human Cardiomyocytes Beauchamp P, Moritz W, Kelm JM, Ullrich ND, Agarkova I, Anson BD, Suter TM, Zuppinger C Tissue Eng Part C Methods. 2015 Aug;21(8):852-61
Novel Xeno-free Human Heart Matrix-derived Three-dimensional Scaffolds Holt-Casper D, Theisen JM, Moreno AP, Warren M, Silva F, Grainger DW, Bull DA, Patel AN J Transl Med. 2015 Jun 18;13:194
Silicon Nanowire-induced Maturation of Cardiomyocytes Derived from Human Induced Pluripotent Stem Cells Tan Y, Richards D, Xu R, Stewart-Clark S, Mani SK, Borg TK, Menick DR, Tian B, Mei Y Nano Lett. 2015 May 13;15(5):2765-72

Interrogation Techniques and Platforms

Title Authors Citation
Human Induced Pluripotent Stem Cell-derived Versus Adult Cardiomyocytes: An In Silico Electrophysiological Study on Effects of Ionic Current Block Paci M, Hyttinen J, Rodriguez B, Severi S Br J Pharmacol. 2015 Nov;172(21):5147-60
A Non-invasive Platform for Functional Characterization of Stem Cell Derived Cardiomyocytes with Applications in Cardiotoxicity Testing Maddah M, Heidmann JD, Mandegar MA, Walker CD, Bolouki S, Conklin BR, Loewke KE Stem Cell Reports. 2015 Apr 14;4(4):621-31
New Easy to Use Hybrid System for Extracellular Potential and Impedance Recordings Doerr L, Thomas U, Guinot DR, Bot CT, Stoelzle-Feix S, Beckler M, George M, Fertig N J Lab Autom. 2015 Apr;20(2):175-88
Automated Patch Clamp Meets High-throughput Screening: 384 Cells Recorded in Parallel on a Planar Patch Clamp Module Obergrussberger A, Brüggemann A, Goetze TA, Rapedius M, Haarmann C, Rinke I, Becker N, Oka T, Ohtsuki A, Stengel T, Vogel M, Steindl J, Mueller M, Stiehler J, George M, Fertig N J Lab Autom. 2015 Dec 23. pii: 2211068215623209. [Epub ahead of print]
Improvement of Acquisition and Analysis Methods in Multi-electrode Array Experiments with iPS Cell-derived Cardiomyocytes Asakura K, Hayashi S, Ojima A, Taniguchi T, Miyamoto N, Nakamori C, Nagasawa C, Kitamura T, Osada T, Honda Y, Kasai C, Ando H, Kanda Y, Sekino Y, Sawada K J Pharmacol Toxicol Methods. 2015 Sep-Oct;75:17-26

Review and Implementation in Toxicity Testing

Title Authors Citation
Human Stem Cell-derived Cardiomyocytes in Cellular Impedance Assays: Bringing Cardiotoxicity Screening to the Front Line Peters MF, Lamore SD, Guo L, Scott CW, Kolaja KL Cardiovasc Toxicol. 2015 Apr;15(2):127-39


Title Authors Citation
Structural and Functional Screening in Human Induced-pluripotent Stem Cell-derived Cardiomyocytes Accurately Identifies Cardiotoxicity of Multiple Drug Types Doherty KR, Talbert DR, Trusk PB, Moran DM, Shell SA, Bacus S Toxicol Appl Pharmacol. 2015 May 15;285(1):51-60
A Multi-parameter In Vitro Screen in Human Stem Cell-derived Cardiomyocytes Identifies Ponatinib-induced Structural and Functional Cardiac Toxicity Talbert DR, Doherty KR, Trusk PB, Moran DM, Shell SA, Bacus S Toxicol Sci. 2015 Jan;143(1):147-55
A Beating Heart Cell Model to Predict Cardiotoxicity: Effects of the Dietary Supplement Ingredients Higenamine, Phenylethylamine, Ephedrine and Caffeine Calvert R, Vohra S, Ferguson M, Wiesenfeld P Food Chem Toxicol. 2015 Apr;78:207-13
Assessment of Cardiomyocyte Contraction in Human-induced Pluripotent Stem Cell-derived Cardiomyocytes Pointon A, Harmer AR, Dale IL, Abi-Gerges N, Bowes J, Pollard C, Garside H Toxicol Sci. 2015 Apr;144(2):227-37
Identification of Genomic Biomarkers for Anthracycline-induced Cardiotoxicity in Human iPSC-derived Cardiomyocytes: An In Vitro Repeated Exposure Toxicity Approach for Safety Assessment Chaudhari U, Nemade H, Wagh V, Gaspar JA, Ellis JK, Srinivasan SP, Spitkovski D, Nguemo F, Louisse J, Bremer S, Hescheler J, Keun HC, Hengstler JG, Sachinidis A Arch Toxicol. 2015 Nov 4. [Epub ahead of print]
High-throughput Cardiac Safety Evaluation and Multi-parameter Arrhythmia Profiling of Cardiomyocytes Using Microelectrode Arrays Gilchrist KH, Lewis GF, Gay EA, Sellgren KL, Grego S Toxicol Appl Pharmacol. 2015 Oct 15;288(2):249-57
High-content Assay Multiplexing for Toxicity Screening in Induced Pluripotent Stem Cell-derived Cardiomyocytes and Hepatocytes Grimm FA, Iwata Y, Sirenko O, Bittner M, Rusyn I Assay Drug Dev Technol. 2015 Nov;13(9):529-46
Cellular Mechanisms for Trazodone-induced Cardiotoxicity Lee S, Lee HA, Kim SJ, Kim KS Hum Exp Toxicol. 2015 Jul 17. pii: 0960327115595683. [Epub ahead of print]
High Throughput Measurement of Ca++ Dynamics in Human Stem Cell-derived Cardiomyocytes by Kinetic Image Cytometery: A Cardiac Risk Assessment Characterization Using a Large Panel of Cardioactive and Inactive Compounds Lu HR, Whittaker R, Price JH, Vega R, Pfeiffer ER, Cerignoli F, Towart R, Gallacher DJ Toxicol Sci. 2015 Dec;148(2):503-16
Proarrhythmia Risk Assessment in Human Induced Pluripotent Stem Cell-derived Cardiomyocytes Using the Maestro MEA Platform Qu Y, Vargas HM Toxicol Sci. 2015 Sep;147(1):286-95
Automated Multi-parameter Measurement of Cardiomyocytes Dynamics with Digital Holographic Microscopy Rappaz B, Moon I, Yi F, Javidi B, Marquet P, Turcatti G. Opt Express. 2015 May 18;23(10):13333-47
An Integrated Platform for Simultaneous Multi-well Field Potential Recording and Fura-2-based Calcium Transient Ratiometry in Human Induced Pluripotent Stem Cell (hiPSC)-derived Cardiomyocytes Rast G, Weber J, Disch C, Schuck E, Ittrich C, Guth BD J Pharmacol Toxicol Methods. 2015 Sep-Oct;75:91-100

Disease Modeling and Rescue

Title Authors Citation
Eag Domains Regulate LQT Mutant hERG Channels in Human Induced Pluripotent Stem Cell-derived Cardiomyocytes Liu QN, Trudeau MC PLoS One. 2015 Apr 29;10(4):e0123951