Phenotypic Drug Discovery Using Primary Human Cells and Co-cultures: Lessons Learned (Nov 12 | 11:30 am EST | Free to SLAS members). Dr. Ellen Berg, BioSeek, describes the features and performance of cell-based assays and co-culture models and summarizes the lessons learned in their use for phenotypic drug discovery applications.
Using Stem Cells to Model Disease (Originally broadcast Sep 19 | Free). Dr. Lawrence Goldstein (University of California-San Diego School of Medicine), Kristen Brennand (Icahn School of Medicine at Mount Sinai), and Lorenz Studer (Sloan-Kettering Center for Stem Cell Biology) discuss how they approach modeling Alzheimer’s disease, schizophrenia, and neurodegenerative disorders using patient-derived iPSCs and how these approaches could be applicable to modeling complex diseases.
The Value of Phenotypic-based Drug Discovery (Originally broadcast Sep 26 | Free to SLAS members). Dr. David Swinney (Institute for Rare and Neglected Diseases Drug Discovery) discusses challenges and solutions to assimilating molecular and phenotypic approaches to increase drug discovery success.
Phenotypic Screening with Human iPS Cell-derived Cardiomyocytes: HTS-compatible Assays for Interrogating Cardiac Hypertrophy. Scientists detail a suite of high-throughput assays focused on BNP detection to show the utility of iCell Cardiomyocytes as an in vitro model of cardiac hypertrophy.
Carlson C, Koonce C, Aoyama N, Einhorn S, Fiene S, Thompson A, Swanson B, Anson B, Kattman S (2013)
J Biomol Screen. Epub ahead of print (DOI: 10.1177/1087057113500812).
Refining the Human iPSC-cardiomyocyte Arrhythmic Risk (hCAR) Assessment Model. Roche researchers optimized an arrhythmia detection assay for use in discovery toxicology and cardiac safety screening. The assay was challenged with 88 marketed and 30 internal compounds and demonstrated that iCell Cardiomyocytes can provide greater predicitivity for QT prolongation and torsadogeneicity in humans.
Guo L, Coyle L, Abrams R, Kemper R, Chiao ET, Kolaja KL (2013) Toxicol Sci. Epub ahead of print
Human-induced Pluripotent Stem Cell-derived Cardiomyocytes Exhibit Temporal Changes in Phenotype. This study demonstrates that iCell Cardiomyocytes maintain their cardiac phenotype in culture without signs of dedifferentiation over time. Under cell culture conditions, iCell Cardiomyocytes acquire adult-like gene expression patterns and functions that are responsive to pathological, physiological, and autonomic pharmacological modulation similar to that of isolated adult cardiac myocytes.
Ivashchenko CY, Pipes GC, Lozinskaya IM, Lin Z, Xiaoping X, Needle S, Grygielko ET, Hu E,
Toomey JR, Lepore JJ, Willette RN (2013) Am J Physiol Heart Circ Physiol. Epub ahead of print