iCell® Cardiomyocytes Genetic Diversity Panel
Investigate Pharmacological Response across a Cohort of Healthy Donors In Vitro
High attrition rates represent a major pitfall for the pharmaceutical industry because current preclinical models do not sufficiently predict drug effects in humans. Critical to these failures are inter-individual variability to drugs. This variability creates a significant need to develop more predictive in vitro models that enable investigation of into inter-individual response. This is ideal for both both safety and efficacy studies within drug development to identify cardiotoxicity early and avoid failures in the clinic.
To enable researchers to evaluate how compounds affect individuals differently and gain more confidence in their findings, FCDI has created the iCell® Cardiomyocytes Genetic Diversity Panel. This reliable, predictive model provides a new solution for more predictive safety and toxicity assessment.
FCDI’s iCell Cardiomyocytes Genetic Diversity Panel are derived from apparently healthy, normal individuals with no known disease-related genotypes. These cardiomyocytes exhibit the relevant biology, diversity, and functionality to advance research and preclinical studies for cardiotoxicity and cardio-oncology.
- ● Fully differentiated, >90% pure cardiomyocytes
- ● Expression of relevant cardiac markers (e.g. cTNT, ACTN2)
- ● Reproducible biology
All lines in the iCell Cardiomyocytes Genetic Diversity Panel are provided with:
- ● Cryopreserved iCell Cardiomyocytes
- ● 30 ml iCell Cardiomyocytes Plating Medium
- ● 100 ml iCell Cardiomyocytes Maintenance Medium
Application protocols for iCell Cardiomyocytes have been optimized for cells derived from the genetic background 01434. A subset of these protocols have been tested with cells derived from the genetic background 11713 and require no modification. Additional applications have not yet been tested using other lines within the panel. Protocols supported for both iCell Cardiomyocytes, 01434 and 11713:
- Field Potential Detection on the Maestro Multielectrode Array
- Impedance Detection with xCELLigence RTCA Cardio System
- Intracellular Calcium Flux Detection on the FLIPR Tetra System
- Immunofluorescent Labeling
- Extracting Total RNA