Part of the iCell Hepatic Portfolio
Hepatoblasts, progenitor cells found in the liver, are defined as endoderm lineage-derived bipotential cells that can differentiate into hepatocytes or cholangiocytes (Figure 1).
Cellular Dynamics has identified the time point in differentiation where endoderm-specific markers are declining and hepatocyte-specific markers are not significantly expressed (Figure 2). This time point is when our commercially available iCell® Hepatoblasts are cryopreserved.
Upon reanimation, iCell Hepatoblasts are ideal for therapeutic research aimed at liver regeneration via the identification of molecules and factors capable of stimulating progenitor cell proliferation/regeneration. They are also perfect for pro/anti hepatocyte differentiation and pro/anti cholangiocyte differentiation assays. In addition, iCell Hepatoblasts can be combined with iCell Endothelial Cells, iCell Hepatocytes, iCell Macrophages, and (available soon) iCell Mesenchymal Stem Cells to engineer complex liver models for in vitro and in vivo applications. These cell combinations enable disease model building by providing access to multiple liver cell types from a single donor for co-culture.
iCell Hepatoblasts retain proliferative capacity in vitro, supplying a dynamic cell population that is driven to differentiate (Figure 3). CDI provides detailed stepwise instructions for thawing, plating, culturing, and differentiating iCell Hepatoblasts into hepatocytes or cholangiocytes.
Contact CDI’S Technical Support to learn more about iCell Hepatoblasts and other ways CDI can further your research through iPS cell technology.