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iCell® Microglia

A Reliable, Pure Source of Human Microglia from iPSC

Description
Performance Data
Cell Images
Documents
Description

Microglia are immune cells resident in the central nervous system (CNS) responsible for fundamental physiological and pathological processes. Microglia support neuronal homeostasis and facilitate neuronal network formation by synaptic pruning. Surveillant microglia can activate, adapt and respond accordingly to specific stimulus. Microglia dysfunction has been implicated in neurodegeneration afflictions, including Parkinson’s and Alzheimer’s diseases, and neurodevelopmental disorders, such as Rett syndrome. The limited availability and inconsistency of primary human microglia has constrained research and therapeutic progress.

 iCell® Microglia are based on technology developed by the Blurton-Jones laboratory (Abud et al. Neuron 2017.) for which FujiFilm Cellular Dynamics Inc. has an exclusive license from the University of California-Irvine.  iCell Microglia overcome the limitations of primary microglial cells by offering a consistent, high-quality microglia derived from human induced pluripotent stem cells, enabling the study of cytokine signaling, synaptic transmission, and plasticity in normal CNS function and during disease progression.

Human Relevance 
iCell Microglia exhibit functional characteristics similar to human microglia, including phagocytosis and cytokine-mediated inflammatory responses, and express relevant microglial markers (TREM2, CX3CR1, TMEM119, P2RY12, IBA1).

Reproducible Research
Rigorous quality control and large-scale manufacturing capabilities ensure consistent performance and reproducible results from every lot of iCell Microglia.

Rapid Results
iCell Microglia are shipped cryopreserved and arrive fully differentiated allowing more experiments to be completed in less time.

 

 

  iCell Microglia, 01279

Sex Male

Age Group (at collection) 55-59 years

Ethnicity Caucasian

Tissue Source PBMC

Reprogramming Method Episomal

Table 1: Donor Background
iCell Microglia, 01279 are derived from a donor with no known disease phenotypes.
Performance Data

Based on published data describing gene expression and signatures of several microglial stages (Abud et al, 2017), iCell Microglia represent a valid in vitro model for CNS research. These cells express TREM2, CXCR4, P2RY12, IBA1, and PU.1 microglial markers at over 80% in the overall cell population. Physiologically, iCell Microglia demonstrate critical responses to stimuli including signature cytokine release, and the phagocytosis of bioparticles.

 

 

Figure 1: iCell Microglia are a Highly Pure Microglial Population.
Flow cytometry analysis of surface markers shows that iCell Microglia exhibit a characteristic microglia signature.


Figure 2: iCell Microglia Secrete Cytokines Upon Stimulation.
iCell Microglia were thawed into 96-well plates and allowed to recover for three days prior to treatment (using lipopolysaccharide (100ng/mL) or IFN gamma (50ng/mL). After 24 hr, cytokine levels in the supernatant were assayed using the Luminex Multiplex Assay. Specific cytokine signatures were observed for each stimulus.


Figure 3: iCell Microglia Phagocytose Bioparticles.
Using the IncuCyte® Live-Cell Analysis System (Sartorius AG), Staphylococcus aureus (SA) BioParticles®  Conjugate (ThermoFisher Scientific) were added to iCell Microglia cultures to assess the kinetics of phagocytosis.

Cell Images

  • Brightfield image of iCell Microglia 1 day post-thaw plated on PEI/laminin coating for adherence (20x).

Documents

  • Ordering InformationFor best pricing please log in or register
    Catalog # Description Quantity Price
    R1131 iCell Microglia Kit, 01279

    Each kit contains:

    • Cryopreserved iCell Microglia, 01279
    • User’s Guide
    ≥1 x 106
    Viable Cells/Vial
    $1350
    Inquire About Pricing & Availability