A Reliable, Pure Source of Human Microglia from iPSC
Microglia are immune cells resident in the central nervous system (CNS) responsible for fundamental physiological and pathological processes. Microglia support neuronal homeostasis and facilitate neuronal network formation by synaptic pruning. Surveillant microglia can activate, adapt and respond accordingly to specific stimulus. Microglia dysfunction has been implicated in neurodegeneration afflictions, including Parkinson’s and Alzheimer’s diseases, and neurodevelopmental disorders, such as Rett syndrome. The limited availability and inconsistency of primary human microglia has constrained research and therapeutic progress.
iCell® Microglia are based on technology developed by the Blurton-Jones laboratory (Abud et al. Neuron 2017.) for which FujiFilm Cellular Dynamics Inc. has an exclusive license from the University of California-Irvine. iCell Microglia overcome the limitations of primary microglial cells by offering a consistent, high-quality microglia derived from human induced pluripotent stem cells, enabling the study of cytokine signaling, synaptic transmission, and plasticity in normal CNS function and during disease progression.
iCell Microglia exhibit functional characteristics similar to human microglia, including phagocytosis and cytokine-mediated inflammatory responses, and express relevant microglial markers (TREM2, CX3CR1, TMEM119, P2RY12, IBA1).
Rigorous quality control and large-scale manufacturing capabilities ensure consistent performance and reproducible results from every lot of iCell Microglia.
iCell Microglia are shipped cryopreserved and arrive fully differentiated allowing more experiments to be completed in less time.
|iCell Microglia, 01279|
|Age Group (at collection)||55-59 years|
|Table 1: Donor Background |
iCell Microglia, 01279 are derived from a donor with no known disease phenotypes.
Based on published data describing gene expression and signatures of several microglial stages (Abud et al, 2017), iCell Microglia represent a valid in vitro model for CNS research. These cells express TREM2, CXCR4, P2RY12, IBA1, and PU.1 microglial markers at over 80% in the overall cell population. Physiologically, iCell Microglia demonstrate critical responses to stimuli including signature cytokine release, and the phagocytosis of bioparticles.
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