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Introducing Neuron Culture Medium

Increase synaptic signaling in iCell® GlutaNeurons with a combination of
BrainPhys™ Neuronal Medium and Wako's Neuron Culture Medium

Figure 1: iCell GlutaNeurons Exhibit Greater Maturation with Wako’s Neuron Culture Medium
iCell GlutaNeurons were plated at 30,000 cells/well and maintained in BrainPhys Neuronal Medium until DIV 3. The medium was then replaced and maintained with either complete BrainPhys or complete BrainPhys combined with Wako’s Neuron Culture Medium. Cells were then treated with a dose curve of the potassium channel blocker 4-aminopyridine. Calcium imaging was performed on the FDSS/μCELL (Hamamatsu Photonics K.K.) using Cal-520® AM calcium dye (ATT Bioquest, Inc.). The results suggest that the combined use of these two media facilitates the generation of spontaneous Ca2+ oscillations in iCell GlutaNeurons through the enhancement of maturation of cell function.

Neuron Culture Medium (FUJIFILM Wako Chemicals), when combined with BrainPhys™ Neuronal Medium (STEMCELL Technologies, Inc.), increases synaptic activity as  measured by calcium bursting in iCell® GlutaNeurons.

Best-in-Class Biology

iCell GlutaNeurons, are a highly pure excitatory neuronal population, expressing AMPA and NMDA that can be modulated using glutamatergic antagonists/agonists, using several types of assays such as calcium signaling and multielectrode arrays. The ability to increase their activity to further develop their maturation in vitro using Wako’s Neuron Culture Medium can enhance the benefits of human neuronal cell modeling as a research tool. Wako’s Neuron Culture Medium is a ready-to-use supplement, capable of inducing rapid neurite outgrowth, using low density cultures and formulated with rat glial supernatant.

iCell GlutaNeurons overcome limitations of existing models by providing the following:

  • Fully differentiated, ≥90% pure population of primarily glutamatergic (excitatory) human neurons
  • Long-term viability, demonstrated reproducibility, and availability in commercial quantities
  • Rapid formation of excitatory neural networks and functional synapses
  • Expression of relevant neurological therapeutic targets and pathways
  • Compatible with a wide range of biochemical and cell-based assays including:
    • Cell viability
    • Calcium signaling
    • Neurite outgrowth and retraction
  • Electrophysiological applications:
    • Identification and characterization of network function
    • Higher throughput assessment of compound efficacy for seizure treatment
    • Higher throughput detection of seizurogenic toxicity

Figure 2: Comparison of BrainPhys to BrainPhys Supplemented with Wako’s Neuron Culture Medium
iCell GlutaNeurons were cultured with BrainPhys alone (Panel A) or BrainPhys+Neuron Culture Medium (9:1, Panel B). The images were captured at DIV 3 weeks, following Ca2+ imaging. Cells were stained with Cal-520 AM calcium dye and imaged using a Yokogawa CQ1 confocal cytometer with 10X objective.

Ordering Information

Vendor Product Name Package Size Catalog Number

FUJIFILM Wako Chemicals Neuron Culture Medium 100 ml 148-09671

FUJIFILM Cellular Dynamics, Inc. iCell GlutaNeurons Kit, 01279 ≥1 x 106
viable cells/vial
R1061

iCell Neural Supplement B 2 ml M1029

iCell Nervous System Supplement 1 ml M1031

STEMCELL Technologies BrainPhys Neuronal Medium 500ml 05790