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MyCell® Cardiomyocytes DCM (L35P)

LMNA-related Dilated Cardiomyopathy

Lamin A/C LMNA mutations are a common cause of dilated cardiomyopathy (DCM). Clinically, DCM is characterized by ventricular dilation concurrent with reduced systolic function and arrhythmias. A missense mutation in the gene encoding the lamin A/C protein (LMNA) results in a change of amino acid 35 from leucine-to-proline (L35P).

To enable researchers to study functional cardiac consequences, somatic cells from an individual carrying the LMNA L35P mutation were reprogrammed to generate MyCell® Cardiomyocytes DCM (L35P), 01016. In addition, an isogenic control, MyCell Cardiomyocytes Corrected DCM (L35P), was generated using genome engineering strategies to correct the mutation. MyCell Cardiomyocytes (L35P) display classic hallmarks of dilated cardiomyopathy including abnormal contractile properties (i.e. reduced contraction amplitude).

Best-in-Class Biology

MyCell Cardiomyocytes (L35P) and its isogenic control (L35P Corrected) exhibit the relevant biology and functionality to advance research and preclinical studies for devastating congenital muscular dystrophies, laminopathies, and cardiomyopathies.

  • Fully differentiated, >90% pure cardiomyocytes
  • Expression of relevant cardiac markers (e.g. cTNT, ACTN2)
  • Isogenic control available

Comparison of MyCell Cardiomyocytes (L35P) and Isogenic Control

Figure 1: Morphology Assessment
Representative phase contrast images (20X) of isogenic control and MyCell Cardiomyocytes (L35P) reveal comparable structure when plated at 20,000 cells per well in 96-well format. (DIV14)

Figure 2: Contractility Measurements (Impedance)
Contractility was monitored by measuring impedance over time. MyCell Cardiomyocytes (L35P) display reduced contraction amplitude relative to isogenic control. (DIV 14)

Figure 3: Calcium Handling Assessment
Representative calcium transients reveal that MyCell Cardiomyocytes (L35P) display increase beat rate relative to isogenic control. (DIV 14)

  • Ordering InformationFor best pricing please log in or register
    Catalog # Description Quantity Price
    DDP-CMC-0.5X 01016.105 MyCell Cardiomyocytes DCM (L35P) Kit, 01016

    Each kit contains:

    • Cryopreserved MyCell L35P Cardiomyocytes
    • 30 ml Plating Medium
    • 2 x 100 ml Maintenance Medium
    • User’s Guide
    ≥2 x 106
    Viable Cells/Vial
    $1350
    Inquire About Pricing & Availability
    DDP-CMC-0.5X 01016.420 MyCell Cardiomyocytes Corrected DCM (L35P) Kit, 01016

    Each kit contains:

    • Cryopreserved MyCell Corrected L35P Cardiomyocytes
    • 30 ml Plating Medium
    • 2 x 100 ml Maintenance Medium
    • User’s Guide
    ≥2 x 106
    Viable Cells/Vial
    $1350
    Inquire About Pricing & Availability