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March 2015

Berger DR, Ware BR, Davidson MD, Allsup SR, and Khetani SR

Enhancing the Functional Maturity of iPSC-derived Human Hepatocytes via Controlled Presentation of Cell-Cell Interactions In Vitro

Hepatology 61(4):1370-81

Publication Date: March 25, 2015

Product Type: iCell Hepatocytes

Summary:

iCell Hepatocytes were cultured in micropatterned islands and co-cultured with mouse fibroblasts to achieve more functionally mature phenotypes including CYP450s (basal and induced), maturity markers (gene expression), and toxin sensitivity.

Impact:

This research highlights the application of iCell Hepatocytes in more complex (organotypic) culture environments and suggests that appropriate culture conditions and signaling mechanisms lead to more mature iCell Hepatocytes.

February 2015

Ware BR, Berger DR, Khetani SR

Prediction of Drug-Induced Liver Injury in Micropatterned Co-cultures Containing iPSC-Derived Human Hepatocytes

Tox Sci 145(2):252-62

Publication Date: February 24, 2015

Product Type: iCell Hepatocytes

Summary:

iCell Hepatocytes were cultured in micropatterned islands and co-cultured with mouse fibroblasts to achieve more functionally mature phenotypes including CYP450s (basal and induced), maturity markers (gene expression), and toxin sensitivity.

Impact:

This research highlights the application of iCell Hepatocytes in more complex (organotypic) culture environments and suggests that appropriate culture conditions and signaling mechanisms lead to more mature iCell Hepatocytes.

Ng S, Schwartz RE, March S, Galstian A, Gural N, Shan J, Prabhu M, Mota MM, Bhatia SN

Human iPSC-derived Hepatocyte-like Cells Support Plasmodium Liver-stage Infection In Vitro

Stem Cell Reports 4(3):348-359

Publication Date: February 7, 2015

Product Type: iCell Hepatocytes

Summary:

iCell Hepatocytes were employed as a model for malaria parasite infection. Cells were infected with Plasmodium falciparum and and Plasmodium vivax species.

Impact:

The study demonstrates the application of iCell Hepatocytes for infectious disease research enabling malaria research for parasitic life cycle modeling as well as drug discovery efforts.

November 2014

Mann DA

Human Induced Pluripotent Stem Cell-derived Hepatocytes for Toxicology Testing

Expert Opin Drug Metab Toxicol 11(1):1-5

Publication Date: November 11, 2014

Product Type: iCell Hepatocytes

Summary:

This editorial describes the current utility of iPSC-derived hepatocytes in toxicological tests, suggesting the shortcomings of stem cell hepatocytes can be overcome by application in organotypic culture models to generate more predictive in vitro assays.

Impact:

iCell Hepatocytes are compatible with and being employed in organotypic culture models. The combination of stem cell-derived hepatocytes and organotypic systems will synergize to generate highly predictive in vitro models.

November 2013

Sirenko O, Hesley J, Rusyn I, and Cromwell EF

High-content Assays for Hepatotoxicity Using Induced Pluripotent Stem Cell-derived Cells

Assay Drug Dev Technol 12(1):43-54

Publication Date: November 14, 2013

Product Type: iCell Hepatocytes

Summary:

iCell Hepatocytes were employed in the screening of a panel of 208 known hepatotoxic and 30 safe compounds by high content analysis for multiple toxic endpoint readouts.

Impact:

The predictive power of the iCell Hepatocytes in assessing compound hepatotoxicity suggests their utility as in vitro assay models for toxicity screening and understanding potential hepatotoxic effects early in the drug development process.