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March 2015

Berger DR, Ware BR, Davidson MD, Allsup SR, and Khetani SR

Enhancing the Functional Maturity of iPSC-derived Human Hepatocytes via Controlled Presentation of Cell-Cell Interactions In Vitro

Hepatology 61(4):1370-81

Publication Date: March 25, 2015

Product Type: iCell Hepatocytes

Summary:

iCell Hepatocytes were cultured in micropatterned islands and co-cultured with mouse fibroblasts to achieve more functionally mature phenotypes including CYP450s (basal and induced), maturity markers (gene expression), and toxin sensitivity.

Impact:

This research highlights the application of iCell Hepatocytes in more complex (organotypic) culture environments and suggests that appropriate culture conditions and signaling mechanisms lead to more mature iCell Hepatocytes.

November 2014

Caralt M, Uzarski JS, Iacob S, Obergfell KP, Berg N, Bijonowski BM, Kiefer KM, Ward HH, Wandinger-Ness A, Miller WM, Zhang ZJ, Abecassis MM, and Wertheim JA

Optimization and Critical Evaluation of Decellularization Strategies to Develop Renal Extracellular Matrix Scaffolds As Biological Templates for Organ Engineering and Transplantation

Am J Transplant 15(1):64-75

Publication Date: November 17, 2014

Product Type: iCell Endothelial Cells

Summary:

iCell Endothelial Cells were used to reform the vasculature in a decellularized rat kidney. The cells were fluorescently tagged to enable visualization of the vascular network.

Impact:

This research is a milestone in the advancement of generating transplantable tissue, either autologous or allogeneic.

Mann DA

Human Induced Pluripotent Stem Cell-derived Hepatocytes for Toxicology Testing

Expert Opin Drug Metab Toxicol 11(1):1-5

Publication Date: November 11, 2014

Product Type: iCell Hepatocytes

Summary:

This editorial describes the current utility of iPSC-derived hepatocytes in toxicological tests, suggesting the shortcomings of stem cell hepatocytes can be overcome by application in organotypic culture models to generate more predictive in vitro assays.

Impact:

iCell Hepatocytes are compatible with and being employed in organotypic culture models. The combination of stem cell-derived hepatocytes and organotypic systems will synergize to generate highly predictive in vitro models.

October 2014

Drawnel FM, Boccardo S, Prummer M, Delobel F, Graff A, Weber M, Gérard R, Badi L, Kam-Thong T, Bu L, Jiang X, Hoflack JC, Kiialainen A, Jeworutzki E, Aoyama N, Carlson C, Burcin M, Gromo G, Boehringer M, Stahlberg H, Hall BJ, Magnone MC, Kolaja K, Chien KR, Bailly J, and Iacone R

Disease Modeling and Phenotypic Drug Screening for Diabetic Cardiomyopathy Using Human Induced Pluripotent Stem Cells

Cell Rep 9(3):810-820

Publication Date: October 30, 2014

Product Type: iCell Cardiomyocytes,

Summary:

Researchers created in vitro models for environmental and genetically-driven diabetic cardiomyopathy. iCell Cardiomyocytes were induced to the cardiomyopathic state through incubation with a diabetic medium while diabetic donor-specific, iPSC-derived MyCell Cardiomyocytes showed the cardiomyopathy phenotype under baseline conditions. A small molecule screen identified molecules that reverted the cardiomyopathy in accordance with the clinical progression of the disease.

Impact:

This research validates using human iPSC-derived technology in phenotypic drug discovery. It demonstrates the recapitulation of cardiomyopathy through environmental and genetic mechanisms and establishes the utility of phenotypic screens to find molecules and pathways that may provide a therapeutic option.