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August 2012

Cerignoli F, Charlot D, Whittaker R, Ingermanson R, Gehalot P, Savchenko A, Gallacher DJ, Towart R, Price JH, McDonough PM, and Mercola M

High Throughput Measurement of Ca2+ Dynamics for Drug Risk Assessment in Human Stem Cell-derived Cardiomyocytes by Kinetic Image Cytometry

J Pharmacol Toxicol Methods 66(3):246-56

Publication Date: August 25, 2012

Product Type: iCell Cardiomyocytes

Summary:

iCell Cardiomyocytes were treated with known cardiotoxic and arrhythmogenic drugs and kinetically analyzed for intracellular calcium concentration ([Ca2+]i). A novel kinetic imaging cytometry (KIC) platform was used to achieve automated, high-resolution, and high-throughput single cell measurement of intracellular fluorescent Ca2+ indicators.

Impact:

iCell Cardiomyocytes showed appropriate biochemical responses to known toxic drug compounds in a high-throughput assay. Data support the use of iCell Cardiomyocytes as a relevant human model system that can be applied in an automated, high-throughput workflow to predict drug-induced cardiotoxicity.

Haythornthwaite A, Stoelzle S, Hasler A, Kiss A, Mosbacher J, George M, Brüggemann A, and Fertig N

Characterizing Human Ion Channels in Induced Pluripotent Stem Cell-derived Neurons

J Biomol Screen 17(9):1264-72

Publication Date: August 24, 2012

Product Type: iCell GABANeurons

Summary:

This characterization study demonstrated that iCell Neurons display cellular electrophysiological properties similar to native human neurons using manual and automated patch clamp recordings.

Impact:

iCell Neurons exhibit electrophysiological characteristics similar to native human neurons and thus provide a relevant in vitro model for disease modeling, drug discovery, and toxicity testing.

Mioulane M, Foldes G, Ali NN, Schneider MD, and Harding SE

Development of High Content Imaging Methods for Cell Death Detection in Human Pluripotent Stem Cell-derived Cardiomyocytes

J Cardiovasc Transl Res 5(5):593-604

Publication Date: August 16, 2012

Product Type: iCell Cardiomyocytes

Summary:

iCell Cardiomyocytes were used in a novel, simple, automated, and scalable fluorescent microscopy assay system to simultaneously measure multiple biochemical endpoints including nuclear remodeling, mitochondrial function, apoptosis, and necrosis.

Impact:

This study demonstrated the use of iCell Cardiomyocytes to assess the effects of drug compounds on multiple cardiac biochemical pathways using an automated high content platform commonly used in the pharmaceutical industry.