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September 2014

Aggarwal P, Turner A, Matter A, Kattman SJ, Stoddard A, Lorier R, Swanson BJ, Arnett DK, and Broeckel U

RNA Expression Profiling of Human iPSC-derived Cardiomyocytes in a Cardiac Hypertrophy Model

PLoS One 9(9):e108051

Publication Date: September 25, 2014

Product Type: iCell Cardiomyocytes

Summary:

Cardiac hypertrophy is characterized by phenotypic, molecular, and genomic changes. This work induced hypertrophy in iCell Cardiomyocytes, demonstrated overlap in transcriptional changes between induced iCell Cardiomyocytes and biopsied cardiac tissue from donor’s with left ventricular hypertrophy, and isolated a unique micro-RNA / mRNA pairing that provided further insight to a complex disease etiology.

Impact:

Most diseases have complex origins and presentations. This work exemplifies the power of iPSC-derived models to recapitulate disease states, elucidate novel molecular entities associated with disease states, and provide insight as to causes and potential therapeutic avenues.

Hayakawa T, Kunihiro T, Ando T, Kobayashi S, Matsui E, Yada H, Kanda Y, Kurokawa J, and Furukawa T

Image-based Evaluation of Contraction–Relaxation Kinetics of Human-induced Pluripotent Stem Cell-derived Cardiomyocytes: Correlation and Complementarity with Extracellular Electrophysiology

J Mol Cell Cardiol 77:178-91

Publication Date: September 23, 2014

Product Type: iCell Cardiomyocytes

Summary:

Researchers used label-free imaging for examining contractility. Cross-platform comparison with electrophysiology, intracellular Ca2+, and traction-force microscopy demonstrated appropriate readout. Functional validation with Na+ channel block, hERG channel block, and beta adrenergic stimulation showed utility for examining cardiomyocyte contraction rate, force, and relaxation.

Impact:

Label-free imaging enables investigators to apply quantitative metrics to iCell Cardiomyocytes’ contractility endpoints in a higher throughput fashion.

Scott CW, Zhang X, Abi-Gerges N, Lamore SD, Abassi YA, and Peters MF

An Impedance-based Cellular Assay Using Human iPSC-derived Cardiomyocytes to Quantify Modulators of Cardiac Contractility

Toxicol Sci 142(2):331-8

Publication Date: September 18, 2014

Product Type: iCell Cardiomyocytes

Summary:

iCell Cardiomyocytes and impedance measurements were shown to be a suitable system for assessing drug-induced effects on contractility. This system was more predictive than rat cardiomyocytes, had assay parameters similar to the gold standard system, and provided higher throughput.

Impact:

This research demonstrates the utility of iCell Cardiomyocytes as a screening model for assessing contractility changes.

Gibson JK, Yue Y, Bronson J, Palmer C, and Numann R

Human Stem Cell-derived Cardiomyocytes Detect Drug-mediated Changes in Action Potentials and Ion Currents

J Pharmacol Toxicol Methods 70(3):255-67

Publication Date: September 16, 2014

Product Type: iCell Cardiomyocytes

Summary:

Researchers recorded action potentials from isolated iCell Cardiomyocytes before and after drug exposure. The recorded responses were stable, sensitive, and robust, demonstrating the appropriate human cardiac electrophysiology and pharmacology.

Impact:

Proarrhythmia prediction can be done at the phenotypic and mechanistic level. This paper complements the growing literature on phenotypic prediction with iCell Cardiomyocytes by demonstrating valid underlying mechanistic processes and pharmacology.

Traister A, Li M, Aafaqi S, Lu M, Arab S, Radisic M, Gross G, Guido F, Sherret J, Verma S, Slorach C, Mertens L, Hui W, Roy A, Delgado-Olguín P, Hannigan G, Maynes JT, and Coles JG

Integrin-linked Kinase Mediates Force Transduction in Cardiomyocytes by Modulating SERCA2a/PLN Function

Nat Commun 5:4533

Publication Date: September 11, 2014

Product Type: iCell Cardiomyocytes

Summary:

Integrin-linked kinase (ILK) associates with protein scaffolding involved in mechanotransduction and contractility. iCell Cardiomyocytes were used to verify ILK as a potential therapeutic target; its modulation altered expression levels and function of other proteins in the scaffold, intracellular Ca2+ signaling, and contractility.

Impact:

This research demonstrates the utility of iCell Cardiomyocytes as a model system for target identification, verification, and screening. A target was identified, its value as a therapeutic target confirmed through over-expression and knockdown, and the functional impact assessed through phenotypic assays.

Belair DG, Whisler JA, Valdez J, Velazquez J, Molenda JA, Vickerman V, Lewis R, Daigh C, Hansen TD, Mann DA, Thomson JA, Griffith LG, Kamm RD, Schwartz MP, and Murphy WL

Human Vascular Tissue Models Formed from Human Induced Pluripotent Stem Cell Derived Endothelial Cells

Stem Cell Rev [Epub ahead of print]

Publication Date: September 5, 2014

Product Type: iCell Endothelial Cells

Summary:

iCell Endothelial Cells were cultured within several bioengineered platforms mimicking the in vivo microenvironment and formed vascular architecture to enable tissue engineering and discovery applications. Assays for measuring migration, invasion, and vascular sprouting in response to cytokines and inhibitors are also reported along with basic characterization data.

Impact:

This is the first iCell Endothelial Cells publication to illustrate multiple applications in bioengineering, vascular network formation, and angiogenesis for regenerative medicine, tissue engineering, toxicity, and discovery applications.