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August 2013

Whitemarsh RC, Tep WH, Bradshaw M, Lin G, Pier CL, Scherf JM, Johnson EA, and Pellett S

Characterization of Botulinum Neurotoxin A Subtypes 1 through 5 by Investigation of Activities in Mice, in Neuronal Cell Cultures, and In Vitro

Infect Immun 81(10):3894-902

Publication Date: August 5, 2013

Product Type: iCell GABANeurons

Summary:

iCell Neurons, along with other commonly used in vitro and in vivo models, were used to characterize the various subtypes of BoNT/A. The results showed distinct in vitro and in vivo toxicological properties between the mouse bioassay (MBA) and in vitro human cell models, thus questioning the predictivity of the MBA.

Impact:

This study supports the need to consider not only the mode of the testing (in vivo versus in vitro) but also the species of the model when designing potency assays for current and future pharmaceutical BoNT preparations. iCell Neurons area relevant human model to detect BoNT.