What plating densities are recommended, in general, for cardiomyocytes? Is this the same for dissociation and patch clamp applications?
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The optimal plating density for iCell Cardiomyocytes can be assay dependent and should be determined empirically based on the intended use. However, a density of ~63,000 plated cardiomyocytes/cm2 provides a beating syncytium and is the recommended starting density for most cell-based assays. See the table below for the recommended number of plated cells for different culture formats.
|Culture Format||Plated Cells||Volume|
|T-25 Flask||1,200,000 – 1,600,000||8 ml|
|6-well Plate||600,000||2 – 3 ml|
|12-well Plate||240,000||1 – 1.2 ml|
|24-well Plate||120,000||500 – 600 µl|
|96-well Plate||20,000||100 µl|
|384-well Plate||5,000||25 – 40 µl|
|1536-well Plate||2,000||8 – 10 µl|
For dissociation applications, we recommend plating at a higher plating density of 750,000 viable cells/well in a 6-well plate according to our Application Protocol.
To obtain single cells that are suitable for manual patch clamp, we recommend plating 40,000 – 80,000 viable cells/well in 2 ml total volume in a 12-well plate containing a coverslip according to our Application Protocol.
- iCell Cardiomyocytes User’s Guide
- Application Protocol: Dissociating Cardiomyocytes by Enzymatic Treatment
- Application Protocol: Measuring Cardiac Electrical Activity: Manual Perforated Patch Clamp