How do I manage edge and environment effects with cardiomyocyte cultures in multiwell plates?
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When culturing iCell® Cardiomyocytes, you may need to adjust conditions to manage edge and environmental effects. Edge effects in the outer wells of a plate related to gas exchange and evaporation, seen as media volume differences, can alter the duration to uniform beating or stabilization of the cell index. To help manage these effects, we recommend filling the outer wells with controls containing medium and dosing vehicle. Environmental effects, related to frequent changes to temperature and CO2 levels in the incubator, also can affect sensitive cells and their measurements. To generate healthy cells and the most stable recordings, store plated cells in the back of a low-traffic incubator set to 37°C and 5% CO2, use incubator separators or an incubator with multiple doors, and double-check that incubator contains water to maintain humidity.