What phenotypic changes are seen with endothelin-1 (ET-1) treatment in cardiomyocytes?
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Carlson et al. (2013) described methods to evaluate the phenotypic change in iCell® Cardiomyocytes cell size in response to ET-1 treatment. These methods include staining actin with phalloidin and evaluating the cell size using image analysis software. Our Application Protocol also provides basic guidelines for immunofluorescent staining using sarcomeric alpha-actinin (ACTN2).
- Application Protocol: Immunofluorescent Labeling
- Carlson et al (2013) Phenotypic screening with human iPS cell-derived cardiomyocytes: HTS-compatible assays for interrogating cardiac hypertrophy.