Which protocol should I use for immunofluorescent labeling of GABANeurons?
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Immunofluorescent labeling is a straightforward technique for assessing the presence and the subcellular localization of an antigen or a protein. Our Application Protocol has demonstrated utility in detecting the presence of microtubule-associated protein 2 (MAP2B), γ-aminobutyric acid (GABA), vesicular GABA transporter (vGAT), vesicular glutamate transporter 2 (vGLUT2), and β-tubulin, class III (tuj-1). This protocol has also shown usefulness in assessing the lack of nestin or glial fibrillary acid protein (GFAP) expression on iCell® GABANeurons.
For more details about neural markers, please see Solution 00000078K, below.
- Application Protocol: Immunofluorescent Labeling