On what days can I transfect and analyze GABANeurons? Which transfection reagent(s) are compatible with your GABANeurons?
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Lipofectamine 2000 (Thermo Fisher Scientific) diluted in Opti-MEM Reduced Serum Medium (Gibco/Thermo Fisher Scientific) and ViaFect Transfection Reagent (Promega) work with iCell® GABANeurons, with approximately 30% transfection efficiency and low toxicity. We prefer to use Lipofectamine 2000 for earlier time points, transfecting on day 4 post-plating and analyzing on day 7 post-plating, and ViaFect reagent at later time points, transfecting on day 21 post-plating and analyzing on day 24 post-plating). A longer post-transfection time has increased efficiency. Also see Solution 00000083G, below.
- Application Note: Applying Transfection Technologies to Create Novel Screening Models
- Application Protocol: Using Liposome-mediated Transfection for Gene Delivery
- Application Protocol: Silencing Gene Expression: siRNA Delivery by Transfection