Do you have a patch clamp protocol for cardiomyocytes?

Do you have a patch clamp protocol for cardiomyocytes?


If you opened this Solution from a search, your last search results are still available in a separate tab. Click the button below to begin a new search.

SEARCH ALL SOLUTIONS

Still Have Questions?
If you did not find an answer to your question, please contact us to speak with an expert:

(877) 320-6688 (toll-free)
+1 (608) 310-5100
or submit a support request

Our Application Protocol demonstrates the utility of and consistency in recording action potentials from iCell® Cardiomyocytes using a manual perforated patch clamp method. This protocol provides instructions for culturing the cardiomyocytes, preparing the perforated patch clamp, identifying suitable cardiomyocytes for patch clamp, filling the recording pipette, forming the gigaohm (GΩ) seal, recording action potentials, preparing saline solutions, managing voltage protocol settings, and washing the glassware.

References:

  1. iCell Cardiomyocytes User’s Guide
  2. Application Protocol: Measuring Cardiac Electrical Activity: Manual Perforated Patch Clamp