How do I need to handle glucose solutions for patch clamping cardiomyocytes?

How do I need to handle glucose solutions for patch clamping cardiomyocytes?


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You need to ensure glucose-containing solutions for iCell® Cardiomyocytes patch clamp methods are filter sterilized and stored at 4°C. We recommend dispensing these solutions into workable aliquots, which are stable up to 1 year when stored at -20°C, according to our Application Protocol. Ma et al. (2011) provided more details about saline solutions for individual ion channels.

For more details about Tyrode’s balanced salt solution, see the related solution 00000065D, below.

References:

  1. iCell Cardiomyocytes User’s Guide
  2. Application Protocol: Measuring Cardiac Electrical Activity: Manual Perforated Patch Clamp
  3. Ma et al (2011) High purity human-induced pluripotent stem cell-derived cardiomyocytes electrophysiological properties of action potentials and ionic currents