What osmolarity and pH values work for patch clamping cardiomyocytes?
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For iCell® Cardiomyocytes, the extracellular saline solution should have an osmolarity of approximately 330 mOsm and a pH of approximately 7.4. The intracellular saline solution should have an osmolarity of approximately 310 mOsm and a pH of approximately 7.2. Glucose can be added to the solution to increase osmolarity.
For more details about handling glucose-containing solutions, see Solution 00000065E.
- iCell Cardiomyocytes User’s Guide
- Application Protocol: Measuring Cardiac Electrical Activity: Manual Perforated Patch Clamp