What general assay guidelines do you have for cardiomyocytes?

What general assay guidelines do you have for cardiomyocytes?


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You can perform most assays after iCell® Cardiomyocytes re-animate from cryopreservation. This re-animation usually occurs about 7 days post-plating, after a confluent monolayer forms and establishes synchronous beating. Cell-beating assays (e.g. impedance and MEA) may benefit from a longer culture of 10 – 14 days. Note that cells are post-mitotic and will not proliferate in culture.

Plating Density

  • Plate cells to enable formation of a confluent monolayer whenever possible.
  • Density can influence gene expression.

Extracellular Matrix (ECM)

  • iCell Cardiomyocytes work with a variety of ECMs.
  • See Solution 00000082A, below, for details about using gelatin and other ECMs.

Media

  • iCell Cardiomyocytes work with a variety of media.
  • Note that ionic concentrations are important for cell beating.
  • Avoid calcium-free media.

Timeline

  • Do not perform assays on freshly thawed (within 5 days) or dissociated cardiomyocytes (within 2 days) whenever possible.
  • For beat rhythm assays, we recommend using cells cultured for 10 – 14 days.

For biochemical and toxicity assays, we recommend using cells cultured for 7 days.

Reference:

  1. iCell Cardiomyocytes User’s Guide

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