What are the purity and population distribution of your neurons?

What are the purity and population distribution of your neurons?


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Each iCell® GABANeurons Kit has >90% purity, as measured by flow cytometry for βIII-tubulin (+) and nestin (-) markers. The mixed population is comprised of approximately 70% GABAergic (inhibitory) and 30% glutamatergic (excitatory) neurons, based on biochemical and electrophysiological data. iCell DopaNeurons are more excitatory, so the balance is shifted towards more glutamatergic populations. Within this population is a sub-fraction of cholinergic receptors, such as the nicotinic acetylcholine receptors (nAChRs), as characterized by Chatzidaki et al. (2015) and Gill et al. (2013).

References:

  1. iCell GABANeurons User’s Guide
  2. iCell GABANeurons Product Page
  3. Chatzidaki et al. (2015) Pharmacological Characterisation of Nicotinic Acetylcholine Receptors Expressed in Human iPSC-derived Neurons
  4. Gill et al. (2013) Contrasting Properties of α7-selective Orthosteric and Allosteric Agonists Examined on Native Nicotinic Acetylcholine Receptors