What markers have you used to characterize iCell GABANeurons?
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Our >90% purity metric for iCell® GABANeurons is based on positive expression of βIII-tubulin and negative expression of nestin, but we also have tested MAP2 (ICC data), synapsin (ICC data), NeuN (microarray data) and several other markers to identify synaptic vesicles (synaptophysin, gephyrin, PSD-95, vGAT, and vGLUT). Gene expression and ICC profiles are reminiscent of cortical neurons. Typical lots are 95 – 98% pure.
The table below provides several common neural markers that can be used for characterization of iCell GABANeurons.
|TU-20, TUBB3||βIII-tubulin, β3-tubulin||microtubule|
|nestin||neuroectodermal stem cell marker||neurofilament, progenitor|
|MAP2||microtubule associated protein 2||microtubule, mature neuron|
|vGAT||vesicular GABA transporter||synaptic vesicles|
|vGluT||vesicular glutamate transporter, glutamate||synaptic vesicles|
|SYP||synaptophysin, major synaptic vesicle protein p38||synaptic vesicles|
|SYN1, SYN2||synapsin I, synapsin II||synaptic vesicles, mature neuron|
|GPHN||gephyrin||synaptic vesicles, neuronal assembly|
|PSD-95, SAP-90||postsynaptic density protein 95, synapse-associated protein 90||synaptic vesicles|
|NeuN||neuronal nuclei||nucleus, cytoplasm, mature neuron|
|TH||tyrosine hydroxylase||dopaminergic neurons|
|GFAP||glial fibrillary acidic protein||astrocytes|
For other characterization profile information, please see Solution 00000091A, below. For more information about immunofluorescent labeling, see Solution 00000061C and our Application Protocol.
- Application Protocol: Immunofluorescent Labeling