What plating density should I use for your endothelial cells?
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We recommend plating iCell® Endothelial Cells at a density of 10,000 – 15,000 cells/cm2. Cells plated at this density are ready to passage after 3 – 4 days. To maintain logarithmic growth, cell passaging should be performed before confluency is reached. Note that we do not recommend plating cells below a density of 10,000 cells/cm2, which may affect proliferation of the cells, or allowing the cells to become confluent, as these cell are contact inhibited. Use the table below as a guideline.
|Culture Vessel||Cells / Well (10,000 – 15,000/cm2)||Well Volume (ml)|
|T-75 Flask||750,000 – 1,125,000||15|
|6-well||96,000 – 144,000||2|
|96-well||32,000 – 48,000||0.2|