What plating density should I use for your endothelial cells?

What plating density should I use for your endothelial cells?


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We recommend plating iCell® Endothelial Cells at a density of 10,000 – 15,000 cells/cm2. Cells plated at this density are ready to passage after 3 – 4 days. To maintain logarithmic growth, cell passaging should be performed before confluency is reached. Note that we do not recommend plating cells below a density of 10,000 cells/cm2, which may affect proliferation of the cells, or allowing the cells to become confluent, as these cell are contact inhibited. Use the table below as a guideline.

Culture Vessel Cells / Well (10,000 – 15,000/cm2) Well Volume (ml)
T-75 Flask 750,000 – 1,125,000 15
6-well 96,000 – 144,000 2
96-well 32,000 – 48,000 0.2

Reference:

  1. iCell Endothelial Cells User’s Guide