What kind of attachment or clumping issues do you see in GABANeurons?
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Attachment of iCell® GABANeurons is not usually an issue on tissue culture plastics when you follow the recommended coating protocols for poly-L-ornithine (PLO) and laminin or poly-D-lysine (PDL) and laminin. However, cells do not adhere easily to glass surfaces. For example, Matrical glass bottom plates, which are useful for imaging and picture quality, show poor cell attachment and irregular (clumped) distribution.
If the laminin coating dries before iCell GABANeurons are plated, adherence may be compromised, and cells may begin to clump and form clusters on the plate. If the neurites become taut and look like spokes, you may be seeing the tendency of iCell GABANeurons to cluster around the <1% astrocytes that may be present.