What feeding schedule do you recommend for cardiomyocytes?
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We recommend feeding iCell® Cardiomyocytes every 48 hours post-plating. Perform 100% medium change from iCell Cardiomyocytes Plating Medium to iCell Cardiomyocytes Maintenance Medium on day 2 post-plating and then perform 100% medium change using Maintenance Medium every 48 hours thereafter.
- Making the first medium change before 48 hours may result in lower plating efficiency.
- Medium change timing can affect beat rate.
- Avoid repeated warming/cooling of the medium stock bottle.
- Dispense the Maintenance Medium into smaller aliquots suitable for each feed (see related solution, below).
Change the medium at least 4 hours before performing experiments; avoid a medium change immediately before experiments.