How do I prepare and store the oncostatin M stock solution used in hepatocyte plating medium?
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For use with iCell® Hepatocytes 2.0, you can prepare 10 μg/ml oncostatin M stock solution in DPBS containing 0.1% BSA and then dispense and store aliquots at -80°C for 3 months. Use oncostatin M (R&D Systems, Cat. No. 295-OM) or an equivalent. Thawed aliquots are stable at 2 – 8°C for 4 weeks. Do not subject aliquots to more than a single refreeze and thaw cycle.
Prepare a 1:500 dilution of stock solution in Plating Medium to a final concentration of 20 ng/ml.