CIRM iPSC Repository

The World's Largest iPSC Bank

The CIRM Induced Pluripotent Stem Cell (iPSC) Repository is a collection of stem cells produced from samples obtained from thousands of individuals. This repository provides researchers with access to cells to study how a disease develops and progresses and to facilitate the discovery and testing of new drugs or other therapies.

The large size of the collection makes it a powerful resource for studying genetic variation between individuals, helping scientists to understand how disease and treatment may vary in a diverse population.

What makes these samples unique?

  • Non-integrating episomal reprogramming
  • Reprogramming and quality control performed by a single entity
  • Majority of donors screened and negative for HIV, HBV, and HCV
  • Demographic and clinical data available

Diseased and Control iPSC Lines Currently Available

Below are the sample counts for the currently available lines in the CIRM iPSC Repository. New donor samples and disease cohorts are added continuously. Thus, for the most accurate sample counts, see the CIRM search tool.

 

Group Disease Donor Samples Control Samples

Neurodevelopmental Disorders Autism Spectrum Disorder 113 175

Cerebral Palsy 19

Intellectual Disability 60

Epilepsy 47

Liver Diseases HCV 93 17

NASH and Steatosis 38

Heart Diseases Cardiomyopathies (Dilated, Hypertrophic, ARVC, LNVC) 442 10

Neurodegenerative Diseases Alzheimer’s Disease 65 100

Blinding Eye Diseases Age-related Macular Degeneration 120

Primary Open Angle Glaucoma 21

Diabetic Retinopathy 34

Lung Diseases Pulmonary Fibrosis 191

How to Use the CIRM Search Tool

Searching the CIRM iPSC Repository

The lines in the CIRM iPSC Repository have been organized into a searchable index. Using the CIRM search tool, you can:

  • Customize your search parameters to identify specific lines that match your research objective.
  • Search using simple text to find lines based on your chosen keyword.
  • See family relationships for available samples.
  • Filter for disease category and further narrow disease type to see only disease-specific lines and controls.
  • Filter for affected status to see either disease samples or their corresponding control lines.
  • Show or hide and sort on any of the sample data parameters.
  • Download the entire data set or selected subset data in your desired file format including .csv (comma-separated values), .xlsx (Excel file format), and PDF. Or simply copy to the clipboard or print your selections.
  • Easily add selections to a cart (including selection from multiple clones where applicable).
  • Transfer all selected lines and quantities to the purchase page and complete the purchase process.

Search, Sort, and Filter Tools

Disease Selection

The CIRM iPSC lines have been grouped by disease category and by subtype where applicable. For example, if you select Liver Disease, you will see only samples and controls for the iPSC lines in the collection that relate to liver diseases. From there, you can select from HCV, NASH, steatosis, or control lines by selecting or deselecting the option check boxes. Where applicable, you can select two disease options to filter samples matching both selections. Options for selection are as follows:


Disease
Type Description

Liver Disease HCV Hepatitis C

NASH Nonalcoholic Steatohepatitis

Steatosis (not NASH)

Controls Unaffected for diseases in this category

Neurodevelopmental Disorders ASD Autism Spectrum Disorder

Cerebral Palsy

Intellectual Disability

Epilepsy

Controls Unaffected for diseases in this category

Cardiomyopathy Hypertrophic

Dilated

Left Ventricular Non-compaction

Arrhythmogenic Right Ventricular

Restrictive

Other See clinical data for these samples

Lung Disease Idiopathic Pulmonary Fibrosis

Other Control samples include self-reported COPD, Asthma, Emphysema

Control Unaffected for diseases in this category

Alzheimer’s/td> Alzheimer’s

Control Unaffected for diseases in this category

Blinding Eye Diseases Age-related Macular Degeneration

Diabetic Retinopathy

Diabetes Type I

Diabetes Type II

Diabetes Type Unknown

Glaucoma/POAG Primary Open-angle Glaucoma

Other See clinical data for these samples

Control Unaffected for diseases in this category

Disease Status

For any set of displayed of iPSC lines, you can filter only for affected, unaffected, or all samples (the default setting).

Note: If you select only control lines using a Disease selector and then choose Affected for Disease Status, you will not see any displayed lines because these two options are mutually exclusive.

Family Status

If you are interested in iPSC lines sampled from sibling or parent/child combinations or are looking for only samples from unrelated individuals, the Family Status drop-down permits the displayed iPSC lines to be filtered on that basis.

Other Selectors

There are other drop-down selectors for Race, Ethnicity, and Sex. These filter the entire set of displayed lines based on the setting selected.

Age Range Sliders

You can narrow the age range for samples based on Age at Sampling, Age at Diagnosis, and Age at Onset.

Note: For any samples where the corresponding data were not provided, the samples will be removed from the displayed lines.

Seeing Familial Relationship Among Lines

When a sample has another family member available within the collection, the family icon (shown right) will be displayed. By clicking this icon (displayed in column 2 under default settings), you can see all related family samples and their relationship. In this example, clicking the Show Related icon for CW60391 reveals two other related samples, CW60391 and CW60389.

Displaying Available Clinical Data

Clicking on the Show Clinical icon (shown right) will reveal the available clinical data for that sample. The complete clinical data sets are available for download here.

Column Visibility and Exporting Data

For any displayed set of lines based on the filters selected, you can export the data to a variety of formats including .csv (comma-separated values), .xlsx (Excel file format), and PDF. Or simply copy the displayed data to the clipboard.

There are many additional columns available beyond what are displayed when the default page loads. You can toggle on or off individual columns for display using the Column visibility drop-down. Any columns set to visible will also be exported as described above.

Adding items to Cart

Clicking on any row will add that line to your cart. The selected row will be highlighted to indicate it has been selected and the corresponding line will appear in the cart information near the top of the page.

You can remove samples from the cart by either clicking the remove icon or clicking on the highlighted item in the table to deselect it.

Note: For some lines, there are more than one clone available. You can select either available clone (or both) by checking the box adjacent to the letter. These individual clones from the same donor were processed in parallel.

Completing and Placing the Order

When you have added all desired items to the cart and are ready to complete the order, click the Order Selected Items button.
Your selected items, along with the desired quantity, will be transferred to the CIRM order form. Because the terms for purchase require a Material Transfer Agreement and for commercial organizations also require a license, the order form initiates the purchase process but does not process payment. After all lines and purchase information have been submitted, FCDI Customer Support will verify any required documentation and contact you to complete the purchase process.

FAQ about the CIRM iPSC Repository

What is the CIRM iPSC Repository?

The CIRM iPSC Repository is a bank of human induced pluripotent stem cells (iPSCs) containing one line from each of approximately 2,500 individuals, 80% of which come from donors having a variety of multigenic disorders and the balance being controls. The donors were selected by leading California researchers to create informative iPSC lines. Donor samples were converted into iPSC lines by FUJIFILM Cellular Dynamics, Inc. (FCDI), using its proprietary episomal reprogramming method that does not introduce exogenous DNA into the iPSC lines. The CIRM iPSC Repository is maintained and administered by FCDI, which is also responsible for storing and distributing de-identified donor clinical information associated with the CIRM iPSC Repository samples.


How can I order a sample from the CIRM iPSC Repository?

Every requester must execute a Material Transfer Agreement with FCDI, which can be downloaded from the ordering webpage. Commercial entities and academics or non-profits engaged in commercial activities must take a patent license from FCDI using the Standard License Agreement for the CIRM iPSC Repository. Commercial activity by an academic or non-profit entity is defined as fee-for-service or sponsored research or other arrangements in which another party has rights to the output of the work performed, even if the work is done by a non-profit.


What are the fees for access to the iPSC lines?

There are two separate fees associated with the CIRM iPSC Repository. There is a fee of $1,500 per vial of iPSCs for a commercial entity and $750 per vial for an academic or non-profit entity. If an entity needs a patent license, a separate fee will be paid to FCDI under the Standard License Agreement.


If I need a patent license, what do I need to do?

A potential requester who wishes to be licensed can print the Standard License Agreement for the CIRM iPSC Repository, execute it by a duly authorized person, and deliver the signed license agreement to FCDI with payment of the initial license fee. The requester is then licensed.


Are the license fees for a single iPSC line or for all the lines in the repository?

The Standard License Agreement for the CIRM iPSC Repository covers all the iPSC lines in the bank. The license fees are not per line but for the entire bank.


Can my organization negotiate any of the terms of the license agreement?

No negotiation of the license terms will be offered for the license fee amounts stated in the Standard License Agreement for the CIRM iPSC Repository. If an organization wants to negotiate any of the terms of the license, FCDI will enter into such negotiations, but additional license fees will apply.


Is there any reach-through claim on the license?

FCDI or CIRM will not claim ownership of a discovery that arises from the use of the iPSC line(s). For example, if the iPSC line(s) or derivatives of them are used in experiments that establish a new small molecule or protein drug candidate, that discovery is owned by the licensee. FCDI has no claim on the discovery. On the other hand, if an activity requires continued use of iPSC line(s), such as making differentiated cells for use in fee-for-hire screening services, that activity requires both a license and the payment of royalties to FCDI under the Standard License Agreement.


If my organization already has a license from iPS Academia Japan (iPS AJ), does my organization pay less fees or get a credit on their fees paid?

iPS AJ has informed FCDI that, as of August 2015, no entity has a license from iPS AJ that is as broad in the research tools space as the license rights available here. Any organization that wishes to explore what credits may be available to it because of an existing license from iPS AJ may contact FCDI, but again additional license fees may apply. On the other hand, no organization should be required to pay royalties twice. If your organization is only doing activities licensed by iPS AJ, an adjustment for royalty fees will be given.


What information is included in the donor consent?

Each donor’s consent included statements on the following:

  • Testing the cells’ DNA and making the information known to other researchers
  • Changing some of the genetic code or sequence within these cells
  • Using cells to test or select drugs to treat disease
  • Transplanting cells or resulting products to humans or animals
  • Distributing cells widely, both nationally and internationally, for research, training, or commercial medical product development
  • Future research and uses unforeseen at this time

What testing is performed on the samples?

Donors were screened for infectious diseases including Human Immunodeficiency Virus (HIV), Hepatitis B (HBV), and Hepatitis C (HCV). Donors under the age of 18 could opt out of HIV testing; all HCV-positive donors submitted skin punches, not peripheral blood, and were not screened for any infectious agents due to small volume of blood present in punches. Additionally, each line undergoes quality control testing that includes:

  • Chromosomal integrity
  • Pluripotency
  • Identity confirmation
  • Loss of reprogramming transgenes
  • Mycoplasma
  • Sterility

How are the samples reprogrammed?

All lines in the CIRM iPSC Repository are created by the same standardized procedure in a single production facility operated by FCDI to minimize lab to lab variability. The samples are reprogrammed by FCDI’s proprietary methods employing non-integrating episomal vectors.


How are the lines culture and cryopreserved?

All lines are grown using Essential 8 Medium and vitronectin (VTN-N) and cryopreserved in colony form.


 

FCDI recommends culturing the iPSC lines using Essential 8 Medium and vitronectin (both available from Thermo Fisher Scientific). Alternatively, the cells can also be cultured using TeSR Medium (available from STEMCELL Technologies) and Matrigel Matrix (available from Corning). It is advised to use the passaging and cryopreservation protocol and reagents recommended by the vendor of the culture medium used.


Is there genomic sequencing or genotype confirmation of mutations in the diseased lines available?

Due to the number of samples in the repository, no genomic or genotyping data have been generated.